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| Genard, Bertrand; Larouche, Olivier; Nicolas, Jean-louis; Miner, Philippe; Beaudin, Marie-lou; Tremblay, Rejean. |
| The aim of this project was to evaluate the impact of probiotic (Phaeobacter gallaeciensis, X34 strain) treatment on the complete development (from veliger to metamorphosis) of Pecten maximus larvae in the context of a bacterial challenge and in conditions more representative of hatchery practices. To that effect, the present study was divided into two main steps. In the first, we used in vitro analyses (antibiograms and microplate assays) to validate the inhibition abilities of X34 on the growth of four Vibrio pathogen species. During the second step, we added pathogens (Vibrio pectenicida) into rearing tanks after two weeks of pre-treatment with the probiotic and then followed the larval development of Pecten maximus through the monitoring of survival... |
| Tipo: Text |
Palavras-chave: Probiotics; Hatchery; Bacterial challenge; Larval development; Pecten maximus; Phaeobacter gallaeciensis; Vibrio. |
| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00213/32429/30895.pdf |
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| Genard, Bertrand; Moraga, Dario; Pernet, Fabrice; David, Elise; Boudry, Pierre; Tremblay, Rejean. |
| Quantification of mRNA of genes related to metabolism, immunity and cellular stress was examined in relation to a massive mortality event during the culture of American oyster larvae, Crassostrea virginica which was probably, in regard to previous microbiological analysis, induced by vibrio infection. To document molecular changes associated with the mortality event, mRNA levels were compared to biochemical and physiological data, previously described in a companion paper. Among the 18 genes studied, comparatively to the antibiotic control, 10 showed a lower relative gene expression when the massive mortality occurred. Six of them are presumed to be related to metabolism, corroborating the metabolic depression associated with the mortality event suggested... |
| Tipo: Text |
Palavras-chave: Massive mortality; Relative gene expression; Metabolism; Immunity; Cellular stress; Larvae. |
| Ano: 2012 |
URL: http://archimer.ifremer.fr/doc/00075/18585/16138.pdf |
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| Genard, Bertrand; Miner, Philippe; Nicolas, Jean-louis; Moraga, Dario; Boudry, Pierre; Pernet, Fabrice; Tremblay, Rejean. |
| Background: Bacterial infections are common in bivalve larvae and can lead to significant mortality, notably in hatcheries. Numerous studies have identified the pathogenic bacteria involved in such mortalities, but physiological changes associated with pathogen exposure at larval stage are still poorly understood. In the present study, we used an integrative approach including physiological, enzymatic, biochemical, and molecular analyses to investigate changes in energy metabolism, lipid remodelling, cellular stress, and immune status of Crassostrea gigas larvae subjected to experimental infection with the pathogenic bacteria Vibrio coralliilyticus. Findings: Our results showed that V. coralliilyticus exposure induced (1) limited but significant increase... |
| Tipo: Text |
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| Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00144/25504/23662.pdf |
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| Genard, Bertrand; Pernet, Fabrice; Lemarchand, Karine; Boudry, Pierre; Moraga, Dario; Tremblay, Rejean. |
| In this paper, biochemical and physiological analyses were used to characterize changes associated with mortality event occurred during veliger development of American oyster, Crassostrea virginica. Biochemical analyses included the evaluation of lipid classes, fatty acid composition and total protein content. Larval physiology was evaluated by studying feeding activity, enzymes related to energy metabolism, oxidative stress levels and enzymatic antioxidant defenses. These analyses were complemented by bacterial community analyses as well as by measuring larval oyster performance. We observed that mortality events coincided with (1) strong changes in the surrounding bacterial community; (2) a progressive decrease in feeding activity; (3) higher levels of... |
| Tipo: Text |
Palavras-chave: Massive mortality; Larvae; Microbial environment; Lipid; Fatty acids; Energy metabolism; Oxidative stress; Antioxidant enzymes; Crassostrea virginica. |
| Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00051/16231/13753.pdf |
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